Abstract
Plant cell wall proteins play essential roles in many important biological processes, and yet there is still not a comprehensive catalogue of the cell wall proteome, or “secretome”. Here, we describe three procedures, including a yeast secretion trap (YST), Agrobacterium-mediated transient expression using a necrosis-inducing protein (NIP) and protein localization assay using a fluorescent protein, to identify and confirm the localization of cell wall proteins. The approaches are orthogonal and collectively provide a powerful suite of approaches to complement more commonly used strategies to isolate plant cell wall-associated proteins.
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Acknowledgements
Research in this area was supported by grants from the NSF Plant Genome Program (DBI-0606595), the New York State Office of Science, Technology and Academic Research (NYSTAR) and Cornell Center for a Sustainable Future (CCSF).
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Lee, SJ., Rose, J.K.C. (2011). Characterization of the Plant Cell Wall Proteome Using High-Throughput Screens. In: Popper, Z. (eds) The Plant Cell Wall. Methods in Molecular Biology, vol 715. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-008-9_18
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DOI: https://doi.org/10.1007/978-1-61779-008-9_18
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