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Characterization of the Plant Cell Wall Proteome Using High-Throughput Screens

  • Sang-Jik Lee
  • Jocelyn K. C. RoseEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 715)

Abstract

Plant cell wall proteins play essential roles in many important biological processes, and yet there is still not a comprehensive catalogue of the cell wall proteome, or “secretome”. Here, we describe three procedures, including a yeast secretion trap (YST), Agrobacterium-mediated transient expression using a necrosis-inducing protein (NIP) and protein localization assay using a fluorescent protein, to identify and confirm the localization of cell wall proteins. The approaches are orthogonal and collectively provide a powerful suite of approaches to complement more commonly used strategies to isolate plant cell wall-associated proteins.

Key words

Plant cell wall protein Secreted protein Secretome, Yeast secretion trap Necrosis-inducing protein Agroinfiltration Confocal fluorescence Extracellular matrix 

Notes

Acknowledgements

Research in this area was supported by grants from the NSF Plant Genome Program (DBI-0606595), the New York State Office of Science, Technology and Academic Research (NYSTAR) and Cornell Center for a Sustainable Future (CCSF).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Plant BiologyCornell UniversityIthacaUSA

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