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New Insights into the Control of Cell Growth

  • Claudia Blaukopf
  • Matthäus Z. Krol
  • Georg J. Seifert
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 715)

Abstract

Undoubtedly, the function of the plant cell wall in the control of cell growth far exceeds its mechanical role. The plant’s monitoring of cell wall function and integrity comprises a central checkpoint to integrate cues for survival and division, expansion and differentiation, as well as fluctuations in the biotic and abiotic environment (Somerville et al., Science 306:2206–2211, 2004). With their biochemical nature yet unknown, the identification of molecular constituents of cell wall performance, and integrity control initially depends on a combination of genetic and physiological approaches.

Key words

Cell wall integrity control Forward genetics Map based cloning Programmed cell death Yariv Arabinogalactan proteins 

Notes

Acknowledgements

We gratefully acknowledge the provision of immunoprobes from Paul Knox and Michael Hahn and sos5 seeds from Jian-Zhang Zhu. We thank Markus Blaukopf for assisting with the preparation of β-Yariv reagent. This work was supported by the Austrian Science foundation FWF grant P19788.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Claudia Blaukopf
    • 1
  • Matthäus Z. Krol
    • 1
    • 2
  • Georg J. Seifert
    • 1
  1. 1.Department of Applied Genetics and Cell BiologyUniversity of Natural Resources and Applied Life SciencesViennaAustria
  2. 2.University of Applied SciencesWiener NeustadtAustria

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