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Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis

  • Derek T. A. LamportEmail author
  • Li Tan
  • Marcia J. Kieliszewski
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 715)

Abstract

Structural proteins of the primary cell wall present unusual but interesting problems for structural biologists in particular and plant biologists in general. As structure is the key to function; then the biochemical isolation of these glycoproteins for further study is paramount. Here, we detail the “classical” method for isolating soluble extensin monomers by elution of monomeric precursors to network extensin from tissue cultures. We also outline an additional approach involving genetic engineering that can potentially yield the complete genomic range of extensins and other hydroxyproline-rich glycoprotein (HRGPs) currently underutilized for biotechnology.

Key words

Extensin Primary cell wall HRGPs AGPs Cultured cells 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Derek T. A. Lamport
    • 1
    Email author
  • Li Tan
    • 2
  • Marcia J. Kieliszewski
    • 3
  1. 1.School of Life SciencesUniversity of SussexBrightonUK
  2. 2.Complex Carbohydrate Research CenterUniversity of GeorgiaAthensUSA
  3. 3.Department of Chemistry and Biochemistry, Biochemistry Research FacilityOhio UniversityAthensUSA

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