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Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organisation of Primary Plant Cell Walls

  • Tracey J. Bootten
  • Philip J. HarrisEmail author
  • Laurence D. Melton
  • Roger H. Newman
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 715)

Abstract

Studies of the mobilities of polysaccharides or parts of polysaccharides in a cell-wall preparation may give clues about the molecular interactions among the polysaccharides in the cell wall and the relative locations of polysaccharides within the cell wall. A number of solid-state 13C NMR techniques have been developed that can be used to investigate different types of polysaccharide mobilities: rigid, semi-rigid, mobile, and highly mobile. In this chapter, techniques are described for obtaining spectra from primary cell-wall preparations using CP/MAS, proton-rotating frame, proton spin-spin, spin-echo relaxation spectra, and single-pulse excitation. We also describe how proton spin relaxation editing can be used to obtain subspectra for cell-wall polysaccharides of different mobilities.

Key words

Primary cell walls Polysaccharide mobility Solid-state 13C NMR Proton-spin relaxation editing Single-pulse excitation NMR TEM X-ray diffraction 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Tracey J. Bootten
    • 1
    • 2
  • Philip J. Harris
    • 1
    Email author
  • Laurence D. Melton
    • 3
  • Roger H. Newman
    • 2
    • 4
  1. 1.School of Biological SciencesThe University of AucklandAucklandNew Zealand
  2. 2.Industrial Research LimitedLower HuttNew Zealand
  3. 3.Food Science, Chemistry DepartmentThe University of AucklandAucklandNew Zealand
  4. 4.ScionRotoruaNew Zealand

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