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The Plant Cell Wall pp 179–196Cite as

Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organisation of Primary Plant Cell Walls

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 715))

Abstract

Studies of the mobilities of polysaccharides or parts of polysaccharides in a cell-wall preparation may give clues about the molecular interactions among the polysaccharides in the cell wall and the relative locations of polysaccharides within the cell wall. A number of solid-state 13C NMR techniques have been developed that can be used to investigate different types of polysaccharide mobilities: rigid, semi-rigid, mobile, and highly mobile. In this chapter, techniques are described for obtaining spectra from primary cell-wall preparations using CP/MAS, proton-rotating frame, proton spin-spin, spin-echo relaxation spectra, and single-pulse excitation. We also describe how proton spin relaxation editing can be used to obtain subspectra for cell-wall polysaccharides of different mobilities.

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Author information

Authors and Affiliations

  1. School of Biological Sciences, The University of Auckland, Auckland, New Zealand

    Tracey J. Bootten & Philip J. Harris

  2. Industrial Research Limited, Lower Hutt, New Zealand

    Tracey J. Bootten & Roger H. Newman

  3. Food Science, Chemistry Department, The University of Auckland, Auckland, New Zealand

    Laurence D. Melton

  4. Scion, Rotorua, New Zealand

    Roger H. Newman

Authors
  1. Tracey J. Bootten
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  2. Philip J. Harris
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  3. Laurence D. Melton
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  4. Roger H. Newman
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Corresponding author

Correspondence to Philip J. Harris .

Editor information

Editors and Affiliations

  1. School of Natural Sciences, Dept. of Botany & Plant Science, National University of Ireland, Galway, University Road, Galway, Ireland

    Zoë A. Popper

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Bootten, T.J., Harris, P.J., Melton, L.D., Newman, R.H. (2011). Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organisation of Primary Plant Cell Walls. In: Popper, Z. (eds) The Plant Cell Wall. Methods in Molecular Biology, vol 715. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-008-9_13

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  • DOI: https://doi.org/10.1007/978-1-61779-008-9_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-007-2

  • Online ISBN: 978-1-61779-008-9

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