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In Vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture

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Plant Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 710))

Abstract

In vitro fertilization (IVF) systems using isolated male and female gametes have been utilized to dissect fertilization-induced events in angiosperms, such as egg activation, zygote development, and early embryogenesis, since the female gametophytes of plants are deeply embedded within ovaries. A rice IVF system was established to take advantage of the abundant resources stemming from rice research for investigations into the mechanisms of fertilization and early embryogenesis. Fusion of gametes can be performed using electrofusion and the fusion product, a zygote, forms a cell wall and an additional nucleolus. The zygote divides into an asymmetric two-celled embryo and develops into an early globular embryo, as in planta. The embryo further develops into irregularly shaped cell masses and fertile plants can be regenerated from the cell masses. This rice IVF system is a powerful tool for studying the molecular mechanisms involved in the early embryogenesis of angiosperms and for making new cultivars.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, Japan

    Takashi Okamoto

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  1. Takashi Okamoto
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Correspondence to Takashi Okamoto .

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Editors and Affiliations

  1. Dept. Biological Sciences, University of Calgary, University Dr. NW., 2500, Calgary, T2N 1N4, Alberta, Canada

    Trevor A. Thorpe

  2. , Department of Biological Sciences, University of Calgary, University Dr. NW 2500, Calgary, T2N 1N4, Alberta, Canada

    Edward C. Yeung

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© 2011 Humana Press

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Okamoto, T. (2011). In Vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture. In: Thorpe, T., Yeung, E. (eds) Plant Embryo Culture. Methods in Molecular Biology, vol 710. Humana Press. https://doi.org/10.1007/978-1-61737-988-8_2

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  • DOI: https://doi.org/10.1007/978-1-61737-988-8_2

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-987-1

  • Online ISBN: 978-1-61737-988-8

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