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Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm

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Plant Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 710))

Abstract

With its beautiful trees, Citrus species have long been valued by humanity. The tasteful fruits, extensively used for nutrition, are also good for health due to the high content in vitamins, minerals, and dietary fibers. Like majority of the woody fruit plants, Citrus germplasm is conserved mainly as field collections in clonal orchards. However, such a traditional approach presents several difficulties, among which are the high cost, manual labor, and extensive land required to maintain the collections, as well as the necessity of a careful protection of plants from diseases and extreme environmental conditions. As many species in the genus have seeds recalcitrant to desiccation, conservation in seed banks is also inadequate. On the other hand, cryopreservation, i.e., the storage of specimens at ultra-low temperatures (usually in liquid nitrogen, at −196°C) where reactions within the cells are minimized, presents a unique alternative for the safe storage of such germplasm. The present contribution outlines the cryopreservation techniques applied to seeds, zygotic and somatic embryos, embryogenic callus cultures of Citrus spp. and provides sample protocols to be used for Citrus conservation.

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Acknowledgment

The procedure under Subheading 3.1 was optimized in the frame of the Project CRIOGERM, financially supported by the Ente Cassa di Risparmio di Firenze, which is gratefully acknowledged.

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Authors and Affiliations

  1. IVALSA/Istituto per la Valorizzazione del Legno e delle Specie Arboree, CNR, Sesto Fiorentino, Firenze, Italy

    Anna De Carlo & Maurizio Lambardi

  2. Biology Department, GYTE, Gebze Institute of Technology, Gebze, Kocaeli, Turkey

    Elif Aylin Ozudogru

Authors
  1. Anna De Carlo
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  2. Maurizio Lambardi
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  3. Elif Aylin Ozudogru
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Corresponding author

Correspondence to Maurizio Lambardi .

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Editors and Affiliations

  1. Dept. Biological Sciences, University of Calgary, University Dr. NW., 2500, Calgary, T2N 1N4, Alberta, Canada

    Trevor A. Thorpe

  2. , Department of Biological Sciences, University of Calgary, University Dr. NW 2500, Calgary, T2N 1N4, Alberta, Canada

    Edward C. Yeung

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© 2011 Humana Press

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De Carlo, A., Lambardi, M., Ozudogru, E.A. (2011). Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm. In: Thorpe, T., Yeung, E. (eds) Plant Embryo Culture. Methods in Molecular Biology, vol 710. Humana Press. https://doi.org/10.1007/978-1-61737-988-8_14

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  • DOI: https://doi.org/10.1007/978-1-61737-988-8_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-987-1

  • Online ISBN: 978-1-61737-988-8

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