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Percutaneous Transendocardial Delivery of Self-Complementary Adeno-Associated Virus 6 in the Canine

  • Lawrence T. Bish
  • Meg M. Sleeper
  • H. Lee Sweeney
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 709)

Abstract

Achieving efficient cardiac gene transfer in a large animal model has proven to be technically challenging. Prior strategies have employed cardio-pulmonary bypass or dual catheterization with the aid of vasodilators to deliver vectors, such as adenovirus, adeno-associated virus (AAV) or plasmid DNA. While single-stranded AAV vectors have shown the greatest promise, they suffer from delayed expression, which might be circumvented by using self-complementary vectors. We have recently optimized a cardiac gene transfer protocol in the canine using a percutaneous transendocardial injection catheter to deliver an AAV vector under fluoroscopic guidance. Percutaneous transendocardial injection of self-complementary AAV (scAAV)-6 is a safe, effective method for achieving efficient cardiac gene transfer to approximately 60% of the myocardium.

Key words

Gene therapy Cardiac Canine model AAV Vector delivery Adeno-associated virus 

Notes

Acknowledgments

This work was funded by a grant from the National Heart, Lung, and Blood Institute (P01-HL059407) (to H.L.S.), the Parent Project Muscular Dystrophy (to H.L.S.), and by T32-HL-007748 (to L.T.B).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Lawrence T. Bish
    • 1
  • Meg M. Sleeper
    • 2
  • H. Lee Sweeney
    • 1
  1. 1.Department of PhysiologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  2. 2.Section of Cardiology, Department of Clinical StudiesVeterinary Hospital of the University of PennsylvaniaPhiladelphiaUSA

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