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Lentiviral Vector Delivery of shRNA into Cultured Primary Myogenic Cells: A Tool for Therapeutic Target Validation

  • Emmanuel Richard
  • Gaelle Douillard-Guilloux
  • Catherine CaillaudEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 709)

Abstract

RNA interference has emerged as a powerful technique to down-regulate gene expression. The lentiviral vector-mediated expression of small hairpin RNAs (shRNAs) from polymerase III promoters allows permanent down-regulation of a specific gene in a wide range of cell types both in vitro and in vivo. In this chapter, we describe a method permitting the expression of shRNA from lentiviral vectors in primary murine myogenic cells. We designed shRNAs targeted to the muscular glycogen synthase isoform (shGYS1), a highly regulated enzyme responsible for glycogen synthesis, in order to modulate the muscle glycogen biosynthetic pathway and to improve the phenotype in primary myogenic cells from a murine model of glycogen storage disease type II (GSDII). This method based on shRNA-mediated down-regulation could be applied to other muscular disorders to evaluate new therapeutic options.

Key words

Lentiviral vector RNA interference Muscular disorder Glycogen synthesis Glycogen synthase Glycogen storage disease Pompe disease Lysosomal storage disorder 

Notes

Acknowledgments

This work was supported by INSERM and the Association Vaincre les Maladies Lysosomales (VML). ER was supported by postdoctoral fellowships from VML and the Association Française contre les Myopathies (AFM). GD was supported by doctoral fellowship from Genzyme (France) and AFM.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Emmanuel Richard
    • 1
  • Gaelle Douillard-Guilloux
    • 1
  • Catherine Caillaud
    • 1
    Email author
  1. 1.INSERM U876, IFR 66, Université Bordeaux 2BordeauxFrance

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