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Recent Developments in Difficult Protein Expression: A Guide to E. coli Strains, Promoters, and Relevant Host Mutations

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Heterologous Gene Expression in E.coli

Part of the book series: Methods in Molecular Biology ((MIMB,volume 705))

Abstract

Escherichia coli is a versatile and popular tool for heterologous protein production. Some of the reasons for its popularity include rapid growth, a variety of portable vectors, relatively simple genetics, and the potential for high-density cultivation. In addition, the extensive laboratory use of E. coli has resulted in technologies to target protein overexpression to various intracellular compartments. This is advantageous because these compartments have different environments that may facilitate folding of particular proteins of interest. This chapter discusses the properties of many of the E. coli strains available for protein expression in order to facilitate the choice of the best expression host for a particular protein of interest.

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Acknowledgments

The author would like to thank Marion Sibley, Elisabeth Raleigh, Paul Riggs, Mehmet Berkmen, Bryce Causey, and Jianying Luo of New England Biolabs for many valuable discussions and for sharing unpublished results. NIH-SBIR grant 1 R43 GM 083413 – 01 has supported work related to the overexpression of membrane proteins.

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Authors and Affiliations

  1. New England Biolabs, Inc., Ipswich, MA, USA

    James C. Samuelson

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  1. James C. Samuelson
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Correspondence to James C. Samuelson .

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Editors and Affiliations

  1. , DNA Enzymes Division, New England Biolabs, Inc., County Road 240, Ipswich, 01938, Massachusetts, USA

    Thomas C. Evans, Jr.

  2. , Chemical Biology Division, New England Biolabs, Inc., County Road 240, Ipswich, 01938, Massachusetts, USA

    Ming-Qun Xu

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Samuelson, J.C. (2011). Recent Developments in Difficult Protein Expression: A Guide to E. coli Strains, Promoters, and Relevant Host Mutations. In: Evans, Jr., T., Xu, MQ. (eds) Heterologous Gene Expression in E.coli. Methods in Molecular Biology, vol 705. Humana Press. https://doi.org/10.1007/978-1-61737-967-3_11

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  • DOI: https://doi.org/10.1007/978-1-61737-967-3_11

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-966-6

  • Online ISBN: 978-1-61737-967-3

  • eBook Packages: Springer Protocols

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