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Ionizing Radiation-Induced DNA Strand Breaks and γ-H2AX Foci in Cells Exposed to Nitric Oxide

  • Kai RothkammEmail author
  • Susanne Burdak-Rothkamm
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 704)

Abstract

A number of studies have demonstrated that nitric oxide enhances radiosensitivity of anoxic and hypoxic cells in vitro and in vivo, and some evidence points to a role for DNA damage and repair in this phenomenon. We have recently observed that nitric oxide enhances the formation of DNA single- and double-strand breaks following ionising irradiation, measured by the alkaline comet assay and immunofluorescence microscopy for γ-H2AX .

Key words

Nitric oxide DNA strand break γ-H2AX single cell gel electrophoresis ionising radiation 

Notes

Acknowledgements

The methods described here were employed in a recent Cancer Research UK-funded study at the Gray Cancer Institute under Peter Wardman’s leadership, with support from Mick Woodcock, Lisa Folkes and Peter Johnston.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Health Protection Agency Centre for Radiation, Chemical & Environmental HazardsOxonUK
  2. 2.Histopathology DepartmentStoke Mandeville HospitalAylesburyUK
  3. 3.Radiation Protection DivisionHealth Protection AgencyDidcotUK

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