Adipose-Derived Stem Cells for Skin Regeneration

  • Hiroshi Mizuno
  • Masaki Nambu
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 702)

Abstract

Intractable skin ulcers resulting from diabetes, ischemia and collagen diseases represent significant problems with few solutions. Cell-based therapy may hold promise in overcoming such disorders. In order to establish a suitable experimental model for the treatment of such ulcers using stem cells, this chapter describes detailed methods for: (1) isolation of stem cells from adipose tissue, termed adipose-derived stem cells (ASCs), (2) preparing a hybrid-type artificial dermis that consists of a type I collagen sponge and ASCs, (3) preparing intractable ulcers using Mitomycin C, and (4) evaluating the effect of wound healing histologically. ASCs seeded onto a type I collagen sponge are applied to intractable ulcers induced by topical application of Mitomycin C. Histological evaluation after 1 and 2 weeks revealed an increase in capillary density and granulation thickness of the hybrid-type artificial dermis. These findings suggest that ASCs may have a positive effect on wound healing and may be a useful tool for future cell-based therapy.

Key words

Wound healing Adipose-derived stem cells Intractable ulcers Angiogenesis Granulation Type I collagen sponge Mitomycin C 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Hiroshi Mizuno
    • 1
  • Masaki Nambu
    • 2
  1. 1.Department of Plastic and Reconstructive SurgerySchool of Medicine, Juntendo UniversityTokyoJapan
  2. 2.Department of Plastic and Reconstructive SurgeryNational Defense Medical CollegeTokorozawaJapan

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