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Three-Dimensional Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells

Part of the Methods in Molecular Biology book series (MIMB,volume 702)

Abstract

Stem cells can easily be harvested from adipose tissue in large numbers for use in tissue-engineering approaches for cartilage repair or regeneration. In this chapter, we describe in vitro tissue-engineering models that we have used in our laboratory for the chondrogenic induction of adipose-derived stem cells (ASC). In addition to the proper growth factor environment, chondrogenesis requires cells to be maintained in a rounded morphology in three-dimensional (3D) culture, and thus properties of the biomaterial scaffold also play a critical role in ASC differentiation. Histologic and immunohistologic methods for assessing chondrogenesis are also presented. In general, 10–12 weeks are required to assess ASC chondrogenesis in these model systems.

Key words

  • Adipose-derived stem cells
  • Chondrogenesis
  • Tissue engineering
  • Alginate
  • Agarose
  • Pellet culture

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Notes

  1. 1.

    Nuclei are stained black or dark blue. Bone, muscles (collagen) are stained green, and cartilage is stained orange or red.

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Acknowledgments

Supported in part by grants from the Duke Translational Medicine Institute, the Coulter Translational Research Partnership, and NIH grants AR50245, AG15768, AR48182, and AR48852. The authors would like to thank Dr. Jeffrey Gimble for many important discussions and collaborations on this work.

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Correspondence to Farshid Guilak .

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Estes, B.T., Guilak, F. (2011). Three-Dimensional Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells. In: Gimble, J., Bunnell, B. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 702. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-960-4_15

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  • DOI: https://doi.org/10.1007/978-1-61737-960-4_15

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