Abstract
Telomeres are highly repetitive sequences at the ends of chromosomes that act as protection structure for chromosome stability. The integration of telomere sequences into the genome by genetic transformation can create chromosome instability because the integrated telomere sequences tend to form de novo telomeres at the site of integration. Thus, telomere repeats can be used to generate minichromosomes by telomere-mediated chromosome truncation in both plants and animals for chromosome studies as well as the applications in genetic engineering as engineered minichromosomes or artificial chromosomes. This protocol describes the procedure for telomere truncation of maize chromosomes by genetic transformation of telomere-containing constructs by both Agrobacterium- and biolistic-mediated transformations.
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Acknowledgments
This protocol was developed in James A. Birchler’s lab at the University of Missouri. The authors would like to thank Professor Birchler for his insightful advice during the protocol development.
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Xu, C., Yu, W. (2011). Telomere Truncation in Plants. In: Birchler, J. (eds) Plant Chromosome Engineering. Methods in Molecular Biology, vol 701. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-957-4_6
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DOI: https://doi.org/10.1007/978-1-61737-957-4_6
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