Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture

  • Justin D. Klein
  • Dario O. Fauza
Part of the Methods in Molecular Biology book series (MIMB, volume 698)


The amniotic fluid and placenta are sources of diverse progenitor cell populations, including ­mesenchymal, hematopoietic, trophoblastic, and possibly more primitive stem cells. Given that much of the amniotic cavity and placenta share a common origin, namely the inner cell mass of the morula, perhaps it is not surprising that most types of progenitor cells that can be isolated from these two sources also share many characteristics. This chapter focuses solely on the most abundant and easy to isolate progenitor cell population found therein, the mesenchymal stem cells (MSCs). Unlike some of the other stem cell types, MSCs are present throughout gestation. Methods of isolation, expansion, freezing, and thawing of these cells will be presented with preference given to the simplest methods available for any given procedure.

Key words

Mesenchymal stem cells Stem cells Amniotic stem cells Amniocytes Mesenchymal amniocytes Amniotic fluid Placenta Fetus Perinatal Tissue engineering Fetal tissue engineering 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of SurgeryChildren’s Hospital Boston and Harvard Medical SchoolBostonUSA

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