Isolation, Culture, and Characterization of Human Umbilical Cord Stroma-derived Mesenchymal Stem Cells

  • Alp Can
  • Deniz Balci
Part of the Methods in Molecular Biology book series (MIMB, volume 698)


As the collection and isolation of human bone marrow-derived mesenchymal stem cells (MSCs) require invasive and often undesirable procurement procedures, investigators have begun to seek alternative sources of human MSC including the umbilical cord stroma. Here we describe the noninvasive isolation, culture, and basic characterization of human umbilical cord stroma-derived mesenchymal stem cells (hUCS-MSCs). Although some technical and observational variations exist between laboratories, there has been a relatively common consensus regarding the immunological and functional characteristics of hUCS-MSCs. Successful in vitro and in vivo differentiation to several lineages makes these cells an invaluable stem cell source, deserving further testing as a cellular therapy or other applications in regenerative medicine. Therefore, the isolation and culture of hUCS-MSCs still need better clarification to ultimately build an optimal standard procedure among laboratories, tissue banks, and clinics.

Key words

Cell culture Cell isolation Mesenchymal stem cell Stem cell Umbilical cord stroma 



The authors would like to thank Drs. Serçin Karahüseyinoğlu and Özgür Çinar who worked as Ph.D. students to improve the described techniques with great pleasure and care. This work was partly supported by The Turkish Ministry of Industry STZ-139-2007-2.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Laboratory for Stem Cell Science, Department of Histology and EmbryologyAnkara University School of Medicine, Ankara University Stem Cell InstituteAnkaraTurkey

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