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Mesenchymal Stem Cell Assays and Applications

  • Mohan C. Vemuri
  • Lucas G. Chase
  • Mahendra S. Rao
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 698)

Abstract

Research on mesenchymal stem cells (MSC) is progressing with increasing popularity. Currently there are a significant number of clinical trials exploring the use of MSCs for the treatment of various diseases including graft-versus-host disease, Crohn’s disease, myocardial infarction, stroke, bone defects, diabetes, and wound repair (www.­clinicaltrails.gov). At the same time, there are questions associated with MSCs in terms of their isolation, culture expansion, phenotype, multipotential differentiation, and transplantation efficiency. This chapter outlines the current status of the field and emphasizes the need for clearly defined protocols to better define the function and use of MSCs in cell therapy.

Key words

MSC Stem cell Self renewal Differentiation Immunophenotype Adipocyte Osteocyte Chondrocyte 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Mohan C. Vemuri
    • 1
  • Lucas G. Chase
  • Mahendra S. Rao
  1. 1.Stem Cells and Regenerative MedicineLife TechnologiesFrederickUSA

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