Abstract
To deal with the data flood of current mass spectrometry methods, standard data formats are needed. The Proteomics Standards Initiative (PSI) of the Human Proteome Organisation (HUPO) develops open storage and transfer standards for and with the community. The Proteomics Informatics work group of the PSI has recently released an XML-based format to store the parameters and results of spectrum identification algorithms (the so-called search engines), which identify peptides and/or proteins from mass spectra. Here, this format called “mzIdentML” is described by giving principle design concepts and presenting examples of important use cases.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Eisenacher M, Martens L, Hardt T et al (2009) Getting a grip on proteomics data – Proteomics Data Collection (ProDaC). Proteomics 9(15):3928–3933
Orchard S, Deutsch EW, Binz PA et al (2009) Annual spring meeting of the Proteomics Standards Initiative. Proteomics 9(19):4429–4432
Taylor CF, Paton NW, Lilley KS et al (2007) The minimum information about a proteomics experiment (MIAPE). Nat Biotechnol 25(8):887–893
Taylor CF, Binz PA, Aebersold R et al (2008) Guidelines for reporting the use of mass spectrometry in proteomics. Nat Biotechnol 26(8):860–861
Binz PA, Barkovich R, Beavis RC et al (2008) Guidelines for reporting the use of mass spectrometry informatics in proteomics. Nat Biotechnol 26(8):862
Stephan C, Kohl M, Turewicz M, Podwojski K, Meyer HE, Eisenacher M. Using Laboratory Information Management Systems as central part of a proteomics data workflow. Proteomics 2010;10:1230–49
Reidegeld KA, Eisenacher M, Kohl M et al (2008) An easy-to-use Decoy Database Builder software tool, implementing different decoy strategies for false discovery rate calculation in automated MS/MS protein identifications. Proteomics 8(6):1129–1137
Beynon RJ, Pratt JM (2005) Metabolic labeling of proteins for proteomics. Mol Cell Proteomics 4(7):857–872
Smith B, Ashburner M, Rosse C et al (2007) The OBO Foundry: coordinated evolution of ontologies to support biomedical data integration. Nat Biotechnol 25(11):1251–1255
Acknowledgments
Martin Eisenacher is funded by CLIB (“Cluster Industrielle Biotechnologie”) project 616 40003 0315413B (Q-ProM). The author wants to thank David Creasy, Andy Jones, Andreas Bertsch, and all other members of the Proteomics Informatics work group for fruitful discussions.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Eisenacher, M. (2011). mzIdentML: An Open Community-Built Standard Format for the Results of Proteomics Spectrum Identification Algorithms. In: Hamacher, M., Eisenacher, M., Stephan, C. (eds) Data Mining in Proteomics. Methods in Molecular Biology, vol 696. Humana Press. https://doi.org/10.1007/978-1-60761-987-1_10
Download citation
DOI: https://doi.org/10.1007/978-1-60761-987-1_10
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60761-986-4
Online ISBN: 978-1-60761-987-1
eBook Packages: Springer Protocols