Production of Tissue-Engineered Skin and Oral Mucosa for Clinical and Experimental Use

  • Sheila MacNeil
  • Joanna Shepherd
  • Louise Smith
Part of the Methods in Molecular Biology book series (MIMB, volume 695)


Since the early 1990s, our understanding of how epithelial and stromal cells interact in 3D tissue-engineered constructs has led to tissue-engineered skin and oral mucosa models, which are beginning to deliver benefit in the clinic (usually in small-scale reconstructive surgery procedures) but have a great deal to offer for in vitro investigations. These 3D tissue-engineered models can be used for a wide variety of purposes such as dermato- and mucotoxicity, wound healing, examination of pigmentation and melanoma biology, and in particular, a recent development from this laboratory, as a model of bacterially infected skin. Models can also be used to investigate specific skin disease processes. In this chapter, we describe the basic methodology for producing 3D tissue-engineered skin and oral mucosa based on de-epidermised acellular human dermis, and we give examples of how these models can be used for a variety of applications.

Key words

Tissue-engineered skin Tissue-engineered oral mucosa Pigmentation Melanoma invasion Infected skin Wound healing 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Sheila MacNeil
    • 1
  • Joanna Shepherd
    • 1
  • Louise Smith
    • 1
  1. 1.Department of Materials Science and Engineering, Kroto Research InstituteUniversity of SheffieldSheffieldUK

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