Abstract
There are several types of bioreactors currently available for the culture of orthopaedic tissue engineered constructs. These vary from the simple to the complex in design and culture. Preparation of samples for bioreactors varies depending on the system being used. This chapter presents data and describes tried and tested methodologies for the preparation of 3D samples for a Rotatory Synthecon Bioreactor (Cellon), a plate shaker, a perfusion system, and a Bose Electroforce Systems Biodynamic Instrument for the in vitro culture of bone and ligament tissue.
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Acknowledgements
The authors wish to acknowledge a private local charity, the Royal Society, and the BBSRC (BBS/S/M/2006/13131 and BB/F013892/1) for financial assistance. The authors also wish to acknowledge the contribution of Mr. Michael Vipin and Dr. Nikki Kuiper (Keele University, UK) for providing the perfusion bioreactor seeding efficiency data. We wish to acknowledge Swann Morton for assistance in gamma sterilisation of the PCL/glass fibre composites and Dr. Alex Fotheringham at Herriot Watt University for plasma sterilisation. We also wish to acknowledge the contribution of the hydroxyapatite scaffold fabrication by Dr. Irene Turner and Dr. Jon Gittings at the University of Bath. Finally, we wish to acknowledge the financial and technical assistance of Mr. David Healy at Giltech Ltd. for the fabrication of the PCL/glass fibre scaffolds.
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Cartmell, S.H., Rathbone, S., Jones, G., Hidalgo-Bastida, L.A. (2011). 3D Sample Preparation for Orthopaedic Tissue Engineering Bioreactors. In: Haycock, J. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 695. Humana Press. https://doi.org/10.1007/978-1-60761-984-0_5
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DOI: https://doi.org/10.1007/978-1-60761-984-0_5
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