Transgene Design

  • Bart van de Sluis
  • Jan Willem Voncken
Part of the Methods in Molecular Biology book series (MIMB, volume 693)


Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and generating a transgenic mouse model are discussed. Methods for DNA purification for microinjection are also provided in this chapter.

Key words

Transgenic Gene expression Promoter DNA construct 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Bart van de Sluis
    • 1
  • Jan Willem Voncken
    • 2
  1. 1.Department of Pathology & Medical Biology, Medical Biology Section, Molecular GeneticsUniversity Medical Center Groningen, University of GronignenGroningenThe Netherlands
  2. 2.Department of Molecular GeneticsMaastricht University Medical CenterMaastrichtThe Netherlands

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