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Transgene Design

  • Bart van de Sluis
  • Jan Willem Voncken
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 693)

Abstract

Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and generating a transgenic mouse model are discussed. Methods for DNA purification for microinjection are also provided in this chapter.

Key words

Transgenic Gene expression Promoter DNA construct 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Bart van de Sluis
    • 1
  • Jan Willem Voncken
    • 2
  1. 1.Department of Pathology & Medical Biology, Medical Biology Section, Molecular GeneticsUniversity Medical Center Groningen, University of GronignenGroningenThe Netherlands
  2. 2.Department of Molecular GeneticsMaastricht University Medical CenterMaastrichtThe Netherlands

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