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Cryopreservation of Mouse Spermatozoa and In Vitro Fertilization

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Transgenic Mouse Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 693))

Abstract

Cryopreservation of mouse spermatozoa has become the foremost technique for preserving large numbers of different strains of mice with induced mutations. Recently, we have established procedures for cryopreservation of mouse spermatozoa and in vitro fertilization using cryopreserved spermatozoa to obtain a relatively high fertilization rate. This chapter attempts to show these procedures in simple terms.

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Nakagata, N. (2011). Cryopreservation of Mouse Spermatozoa and In Vitro Fertilization. In: Hofker, M., van Deursen, J. (eds) Transgenic Mouse Methods and Protocols. Methods in Molecular Biology, vol 693. Humana Press. https://doi.org/10.1007/978-1-60761-974-1_4

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  • DOI: https://doi.org/10.1007/978-1-60761-974-1_4

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  • Print ISBN: 978-1-60761-973-4

  • Online ISBN: 978-1-60761-974-1

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