Adenovirus-Mediated Gene Transfer

  • Ko Willems van Dijk
  • Kyriakos E. Kypreos
  • Frits J. Fallaux
  • Jurre HagemanEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 693)


Recombinant adenoviruses are attractive vectors for short-term expression in mouse liver and primary cell lines. Various versatile vector systems have been developed which can be used for the reliable production of recombinant adenoviruses. This protocol describes the entire process for the production of recombinant adenoviruses using the AdEasy system. This protocol will give a practical step-by-step description from the cloning of the gene of interest until the in vivo administration in mice. The entire process will take about 8 weeks to complete.

Key words

Recombinant adenoviruses Adenovirus AdEasy pAdEasy Viral transduction Transduction 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Ko Willems van Dijk
    • 1
  • Kyriakos E. Kypreos
    • 2
  • Frits J. Fallaux
    • 3
  • Jurre Hageman
    • 4
    Email author
  1. 1.Departments of Human and Clinical GeneticsLeiden University Medical CenterLeidenThe Netherlands
  2. 2.Pharmacology Unit, Department of MedicineUniversity of Patras Medical SchoolRioGreece
  3. 3.Utrecht HoldingsUniversity of UtrechtUtrechtThe Netherlands
  4. 4.Laboratory of Pediatrics, Digestive and Metabolic Diseases Center for LiverUniversity Medical Center Groningen, University of GroningenGroningenThe Netherlands

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