Genetic Modification of the Mouse: General Technology – Pronuclear and Blastocyst Injection

  • Jan Willem VonckenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 693)


Introduction of germ line mutations in mice via genetic engineering involves alterations of the structure and characteristics of genes. These alterations are mostly introduced via molecular genetic technology either in embryonal stem cells or in one-cell stage embryos. This chapter describes classic biotechnological methods used to generate mice from modified pre-implantation embryos.

Key words

Transgenic Gene expression Promoter DNA construct 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Molecular GeneticsMaastricht University Medical CenterMaastrichtThe Netherlands

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