Advertisement

Genetic Modification of the Mouse: General Technology – Pronuclear and Blastocyst Injection

  • Jan Willem VonckenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 693)

Abstract

Introduction of germ line mutations in mice via genetic engineering involves alterations of the structure and characteristics of genes. These alterations are mostly introduced via molecular genetic technology either in embryonal stem cells or in one-cell stage embryos. This chapter describes classic biotechnological methods used to generate mice from modified pre-implantation embryos.

Key words

Transgenic Gene expression Promoter DNA construct 

References

  1. 1.
    Hogan, B., Beddington, R., Constantini, F., and Lacey, E. (1994) Manipulating the mouse embryo. A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
  2. 2.
    Pedersen, R. A., and Rossant, J. (1989) Transgenic techniques in mice: a video guide. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
  3. 3.
    Pieper, F. R., et al. (1992) Efficient generation of functional transgenes by homologous recombination in murine zygotes. Nucleic Acids Res 20, 1259–1264.PubMedCrossRefGoogle Scholar
  4. 4.
    Sigmund, C. D. (1993) Major approaches for generating and analyzing transgenic mice. An overview. Hypertension 22, 599–607.PubMedGoogle Scholar
  5. 5.
    Bronson, S. K., et al. (1996) Single-copy transgenic mice with chosen-site integration. Proc Natl Acad Sci USA 93, 9067–9072.PubMedCrossRefGoogle Scholar
  6. 6.
    Sambrook, J., Fritsch E. F., and Maniatis, T. (1989) Molecular cloning. A laboratory manual. 2nd ed. (Ford, N., ed.), Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
  7. 7.
    Taketo, M., et al. (1991) FVB/N: an inbred mouse strain preferable for transgenic analyses. Proc Natl Acad Sci USA 88, 2065–2069.PubMedCrossRefGoogle Scholar
  8. 8.
    Laird, P. W., et al. (1991) Simplified mammalian DNA isolation procedure. Nucleic Acids Res 19, 4293.PubMedCrossRefGoogle Scholar
  9. 9.
    Chen, S. Z., and Evans, G. A. (1990) A simple screening method for transgenic mice using the polymerase chain reaction. Biotechniques 8, 32–33.PubMedGoogle Scholar
  10. 10.
    Ohhara, M., Kurosu, Y., and Esumi, M. (1994) Direct PCR of whole blood and hair shafts by microwave treatment. Biotechniques 17, 726–728.Google Scholar
  11. 11.
    Malumbres, M., et al. (1997) Isolation of high molecular weight DNA for reliable genotyping of transgenic mice. Biotechniques 22, 1114–1119.PubMedGoogle Scholar
  12. 12.
    Henneberger, C., Grantyn, R., and Rothe, T. (2000) Rapid genotyping of newborn gene mutant mice. J Neurosci Meth 100, 123–126.CrossRefGoogle Scholar
  13. 13.
    Cases, O., et al. (1995) Aggressive behavior and altered amounts of brain serotonin and norepinephrine in mice lacking MAOA. Science 268, 1763–1766.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Molecular GeneticsMaastricht University Medical CenterMaastrichtThe Netherlands

Personalised recommendations