Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells

  • Menno P. J. de WintherEmail author
  • Peter Heeringa
Part of the Methods in Molecular Biology book series (MIMB, volume 693)


Immune cells are derived from hematopoietic stem cells in the bone marrow. Experimental replacement of bone marrow offers the unique possibility to replace immune cells, to study gene function in mouse models of disease. Over the past decades, this technique has been used extensively to study, for instance, macrophage function in atherosclerosis. In this chapter, we describe the methods for both full bone marrow transplantations and T cell-specific transfers.

Key words

Immune cells Macrophages Bone marrow transplantation T cells Mouse Atherosclerosis 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Molecular Genetics, Cardiovascular Research Institute MaastrichtMaastricht UniversityMaastrichtThe Netherlands
  2. 2.Department of Pathology & Medical Biology, Medical Biology SectionUniversity Medical Center Groningen, University of GroningenGroningenThe Netherlands

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