MICER Targeting Vectors for Manipulating the Mouse Genome
The mouse has become an important model for understanding human development, physiology and disease because of its genetic and biological similarity to humans. Desired mouse mutants with precise genetic alterations can now be generated through gene targeting in mouse embryonic stem cells. The rate-limiting factor in a gene-targeting experiment is the time needed for cloning to construct targeting vectors. The establishment of the Mutagenic Insertion and Chromosome Engineering Resource has made available targeting vectors for the insertional mutagenesis of a large number of mouse genes as well as for chromosome engineering throughout the mouse genome. This unique resource has enriched the repertoire of the genetic reagents for targeted manipulation of the mouse genome.
Key wordsMICER Gene targeting Targeting vectors Insertional mutagenesis Chromosome engineering
We thank Annie Pao and Paula Jones for their assistance. Work in the authors’ laboratory is supported by grants from the Louis Sklarow Memorial Fund, the Jerome Lejeune Foundation and the National Institutes of Health (R01HL91519).
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