Abstract
High-performance liquid chromatography (HPLC) coupled in-line with mass spectrometry (MS) permits rapid and specific identification and quantification of N-acyl-l-homoserine lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs). We are presenting here methods for the analysis of these molecules directly from biological samples using LC/MS.
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Acknowledgments
The important contribution of Sylvain Milot to the development of these methods is gratefully acknowledged. We also thank Émilie Gauthier for the help in the development of the AHLs quantification methods.
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Lépine, F., Déziel, E. (2011). Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of N-Acyl-l-Homoserine Lactones and 4-Hydroxy-2-Alkylquinolines. In: Rumbaugh, K. (eds) Quorum Sensing. Methods in Molecular Biology, vol 692. Humana Press. https://doi.org/10.1007/978-1-60761-971-0_5
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DOI: https://doi.org/10.1007/978-1-60761-971-0_5
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