Quorum Sensing pp 219-233 | Cite as

Linking Quorum Sensing Regulation and Biofilm Formation by Candida albicans

  • Aurélie Deveau
  • Deborah A. Hogan
Part of the Methods in Molecular Biology book series (MIMB, volume 692)


Candida albicans biofilms are surface-associated, structured communities composed of yeast, hyphal, and pseudohyphal cells surrounded by an extracellular matrix. C. albicans biofilms often lead to life-threatening systemic infections and are particularly difficult to eradicate because of their high levels of resistance to antibiotics. Farnesol, an autoregulatory molecule secreted by C. albicans, inhibits hyphal growth and the expression of a number of morphology-specific genes that are necessary for robust biofilm formation. Many stages of biofilm development are impacted by farnesol including the adherence of cells to the substratum, the architecture of mature biofilms, and the dispersal of cells from biofilms. For these reasons, understanding the mechanisms of action of farnesol could lead to the development of new antifungal compounds that target C. albicans biofilm cells, perhaps rendering biofilms more sensitive to antibiotics. Here, we describe several methods for the analysis of the effects of farnesol on biofilm formation and function.

Key words

Farnesol Biofilm Candida albicans Morphology 



We thank Dr. Aaron Mitchell (Carnegre mellon University, PA, USA) for providing CSLM pictures. The data described in this chapter were generated in studies funded by NIH (K22 DE016542, D.A.H.).


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Aurélie Deveau
    • 1
  • Deborah A. Hogan
    • 1
  1. 1.Department of Microbiology and ImmunologyDartmouth Medical SchoolHanoverUSA

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