Analysis of Glycosaminoglycans in Stem Cell Glycomics

  • Boyangzi Li
  • Haiying Liu
  • Zhenqing Zhang
  • Hope E. Stansfield
  • Jonathan S. Dordick
  • Robert J. Linhardt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 690)

Abstract

Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by profiling for the GAG content of the differentiated cells. Here, embryonic-like teratocarcinoma cells, NCCIT, a developmentally pluripotent cell line, were used as a model for establishing GAG glycomic methods, but will be easily transferrable to embryonic stem cell cultures.

Key words

Glycosaminoglycans NCCIT cells Chondroitin sulfate Dermatan sulfate Heparin Heparan sulfate Purification Enzymatic digestion Disaccharide analysis LC-MS 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Boyangzi Li
    • 1
  • Haiying Liu
    • 2
  • Zhenqing Zhang
    • 1
  • Hope E. Stansfield
    • 3
  • Jonathan S. Dordick
    • 4
  • Robert J. Linhardt
    • 5
  1. 1.Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA
  2. 2.Department of Biology, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA
  3. 3.Departments of Chemistry and Chemical Biology and Biology, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA
  4. 4.Departments of Biology and Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA
  5. 5.Department of Chemistry and Chemical Biology and Department of Biology and Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA

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