Skip to main content

Deconstructing Adult Zebrafish Behavior with Swim Trace Visualizations

  • Protocol
  • First Online:
Zebrafish Neurobehavioral Protocols


Three-dimensional reconstruction is a method of qualifying the behavioral activity of several animals including mice, rats, and zebrafish. This method allows for measuring behavioral endpoint data on two types of tracking planes (temporal and spatial). Temporal tracking measures the activity of a subject across time while spatial tracking measures the activity of a subject in a specific area of the experimental arena as such. Data representation over 3D visual trajectory reconstruction is a robustly advantageous method of behavioral phenotyping. Digital video-tracking and analysis eliminates the inaccuracies of manual tracking and allows for improved investigation of behavioral activity at specific points in time or specific areas of the tracking arena.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others


  1. Stamou, G. et al. 2D and 3D Motion Tracking in Digital Video in Handbook of Image and Video Processing (ed. A.C. Bovik) (Elsevier Academic Press, Burlington, 2005).

    Google Scholar 

  2. Noldus, L.P.J.J., Spink, A.J., & Tegelenbosch, R.A.J. Computerised video tracking, movement analysis and behaviour recognition in insects. Comput. Electron. Agric. 35(2–3), 201–227 (2002).

    Article  Google Scholar 

  3. Masson, G.S. From 1D to 2D via 3D: dynamics of surface motion segmentation for ocular tracking in primates. J. Physiol. (Paris) 98(1–3), 35–52 (2004).

    Article  Google Scholar 

  4. Noldus, L.P., Spink, A.J., & Tegelenbosch, R.A. EthoVision: a versatile video tracking system for automation of behavioral experiments. Behav. Res. Methods Instrum. Comput. 33(3), 398–414 (2001).

    Article  PubMed  CAS  Google Scholar 

  5. Kane, A.S. et al. A video-based movement analysis system to quantify behavioral stress responses of fish. Water Res. 38(18), 3993–4001 (2004).

    Article  PubMed  CAS  Google Scholar 

  6. Fontaine, E. et al. Automated visual tracking for studying the ontogeny of zebrafish swimming. J. Exp. Biol. 211(Pt 8), 1305–1316 (2008).

    Article  PubMed  Google Scholar 

  7. Delcourt, J. et al. A video multitracking system for quantification of individual behavior in a large fish shoal: advantages and limits. Behav. Res. Methods 41(1), 228–235 (2009).

    Article  PubMed  Google Scholar 

  8. Cachat, J.M. et al. Video-aided analysis of zebrafish locomotion and anxiety-related behavioral responses in Zebrafish Neurobehavioral Protocols (eds. A.V. Kalueff & J. Cachat) (Humana Press, New York, NY, 2010).

    Google Scholar 

  9. Grossman, L. et al. Characterization of behavioral and endocrine effects of LSD on zebrafish. Behav. Brain Res. 214, 277–284 (2010).

    Article  PubMed  CAS  Google Scholar 

Download references


The study was supported by Tulane University Intramural Research program, Provost’s Scholarly Enrichment Fund, Lurcy Fellowships, LA Board of Regents P-Fund, Newcomb Fellows Grant, and Zebrafish Neuroscience Research Consortium (ZNRC).

Author information

Authors and Affiliations


Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Cachat, J.M. et al. (2011). Deconstructing Adult Zebrafish Behavior with Swim Trace Visualizations. In: Kalueff, A., Cachat, J. (eds) Zebrafish Neurobehavioral Protocols. Neuromethods, vol 51. Humana Press.

Download citation

  • DOI:

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-952-9

  • Online ISBN: 978-1-60761-953-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics