Abstract
Three-dimensional reconstruction is a method of qualifying the behavioral activity of several animals including mice, rats, and zebrafish. This method allows for measuring behavioral endpoint data on two types of tracking planes (temporal and spatial). Temporal tracking measures the activity of a subject across time while spatial tracking measures the activity of a subject in a specific area of the experimental arena as such. Data representation over 3D visual trajectory reconstruction is a robustly advantageous method of behavioral phenotyping. Digital video-tracking and analysis eliminates the inaccuracies of manual tracking and allows for improved investigation of behavioral activity at specific points in time or specific areas of the tracking arena.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Stamou, G. et al. 2D and 3D Motion Tracking in Digital Video in Handbook of Image and Video Processing (ed. A.C. Bovik) (Elsevier Academic Press, Burlington, 2005).
Noldus, L.P.J.J., Spink, A.J., & Tegelenbosch, R.A.J. Computerised video tracking, movement analysis and behaviour recognition in insects. Comput. Electron. Agric. 35(2–3), 201–227 (2002).
Masson, G.S. From 1D to 2D via 3D: dynamics of surface motion segmentation for ocular tracking in primates. J. Physiol. (Paris) 98(1–3), 35–52 (2004).
Noldus, L.P., Spink, A.J., & Tegelenbosch, R.A. EthoVision: a versatile video tracking system for automation of behavioral experiments. Behav. Res. Methods Instrum. Comput. 33(3), 398–414 (2001).
Kane, A.S. et al. A video-based movement analysis system to quantify behavioral stress responses of fish. Water Res. 38(18), 3993–4001 (2004).
Fontaine, E. et al. Automated visual tracking for studying the ontogeny of zebrafish swimming. J. Exp. Biol. 211(Pt 8), 1305–1316 (2008).
Delcourt, J. et al. A video multitracking system for quantification of individual behavior in a large fish shoal: advantages and limits. Behav. Res. Methods 41(1), 228–235 (2009).
Cachat, J.M. et al. Video-aided analysis of zebrafish locomotion and anxiety-related behavioral responses in Zebrafish Neurobehavioral Protocols (eds. A.V. Kalueff & J. Cachat) (Humana Press, New York, NY, 2010).
Grossman, L. et al. Characterization of behavioral and endocrine effects of LSD on zebrafish. Behav. Brain Res. 214, 277–284 (2010).
Acknowledgments
The study was supported by Tulane University Intramural Research program, Provost’s Scholarly Enrichment Fund, Lurcy Fellowships, LA Board of Regents P-Fund, Newcomb Fellows Grant, and Zebrafish Neuroscience Research Consortium (ZNRC).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Cachat, J.M. et al. (2011). Deconstructing Adult Zebrafish Behavior with Swim Trace Visualizations. In: Kalueff, A., Cachat, J. (eds) Zebrafish Neurobehavioral Protocols. Neuromethods, vol 51. Humana Press. https://doi.org/10.1007/978-1-60761-953-6_16
Download citation
DOI: https://doi.org/10.1007/978-1-60761-953-6_16
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60761-952-9
Online ISBN: 978-1-60761-953-6
eBook Packages: Springer Protocols