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Light Microscopy pp 51–68Cite as

Image Cytometry: Nuclear and Chromosomal DNA Quantification

Part of the Methods in Molecular Biology book series (MIMB,volume 689)

Abstract

Image cytometry (ICM) associates microscopy, digital image and software technologies, and has been particularly useful in spatial and densitometric cytological analyses, such as DNA ploidy and DNA content measurements. Basically, ICM integrates methodologies of optical microscopy calibration, standard density filters, digital CCD camera, and image analysis softwares for quantitative applications. Apart from all system calibration and setup, cytological protocols must provide good slide preparations for efficient and reliable ICM analysis. In this chapter, procedures for ICM applications employed in our laboratory are described. Protocols shown here for human DNA ploidy determination and quantification of nuclear and chromosomal DNA content in plants could be used as described, or adapted for other studies.

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Acknowledgments

We thank CNPq – Conselho Nacional de Desenvolvimento Científico e Tecnológico and CBP&D/Café – Consórcio Brasileiro de Pesquisa e Desenvolvimento do Café, Brazil, for their financial support.

Author information

Authors and Affiliations

  1. Laboratory of Cytogenetic and Cytometry, Department of General Biology, Federal University of Viçosa, Viçosa, MG, Brazil

    Carlos Roberto Carvalho, Wellington Ronildo Clarindo & Isabella Santiago Abreu

Authors
  1. Carlos Roberto Carvalho
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  2. Wellington Ronildo Clarindo
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  3. Isabella Santiago Abreu
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Corresponding author

Correspondence to Carlos Roberto Carvalho .

Editor information

Editors and Affiliations

  1. Laboratory of Structural Biology &, Reproduction, Federal University of Minas Gerais, Av Antonio Carlos- Pampulha 6627, Belo Horizonte, 31270-901, Minas Gerais, Brazil

    Hélio Chiarini-Garcia

  2. Laboratory of Cellular Biology, Department of Biology, ICB, Federal University of Juiz de Fora, Campus 36.036-330, Juiz de Fora, MG, 36036-900, Brazil

    Rossana C. N. Melo

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© 2011 Humana Press

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Carvalho, C.R., Clarindo, W.R., Abreu, I.S. (2011). Image Cytometry: Nuclear and Chromosomal DNA Quantification. In: Chiarini-Garcia, H., Melo, R. (eds) Light Microscopy. Methods in Molecular Biology, vol 689. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-950-5_4

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  • DOI: https://doi.org/10.1007/978-1-60761-950-5_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-949-9

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