Abstract
Methods to isolate and purify 6- and 5-Chl D1/D2/Cyt b559 photosystem II (PSII) reaction center (RC) complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material (i.e., more nonpigmented protein) is also described. Criteria to assess the purity of PSII RC preparations are presented, and problems associated with each of the isolation procedures are discussed.
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Acknowledgments
The authors would like to thank all of their current and past collaborators for helping us make good use of the preparations described in this article. This work was supported by the Chemical Sciences, Geosciences, and Biosciences Division, Office of Science, U.S. Department of Energy (MS), and by the MICINN of Spain (Grant BFU2005-04722-C02-01 and AGL2008-00377) to RP.
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Seibert, M., Picorel, R. (2011). Isolation of Photosystem II Reaction Center Complexes from Plants. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods in Molecular Biology, vol 684. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-925-3_3
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DOI: https://doi.org/10.1007/978-1-60761-925-3_3
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