Application of a Fusiogenic Peptide GALA for Intracellular Delivery

  • Ikuhiko Nakase
  • Kentaro Kogure
  • Hideyoshi Harashima
  • Shiroh FutakiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 683)


To enhance the cytosolic delivery of therapeutic drugs and genes, pH-sensitive and membrane fusiogenic peptides have been employed as additives for facilitating their endosomal escape. GALA is such a peptide composed of repeating sequences of Glu-Ala-Leu-Ala, which are designed to mimic the function of viral fusion protein sequences that mediate escape of virus genes from acidic endosomes to the cytosol. Recently, the peptide has been applied not only for improving the transfection efficiency of plasmid DNAs using cationic liposomes, but also as functional molecules of multifunctional envelope-type nanodevice (MEND). The advantage of employing this peptide was also exemplified by the cytosolic delivery of proteins via efficient endosomal escape of the GALA–cargo conjugates in the presence of cationic lipid complexes. This chapter provides protocols for the efficient cytosolic delivery of cargo molecules using the GALA peptide.

Key words

Cytosolic delivery GALA Membrane fusiogenic peptide Cationic liposome Endosomal escape 



This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Ikuhiko Nakase
    • 1
  • Kentaro Kogure
    • 2
  • Hideyoshi Harashima
    • 3
  • Shiroh Futaki
    • 1
    Email author
  1. 1.Institute for Chemical ResearchKyoto UniversityKyotoJapan
  2. 2.Department of Biophysical ChemistryKyoto Pharmaceutical UniversityKyotoJapan
  3. 3.Faculty of Pharmaceutical SciencesHokkaido UniversitySapporoJapan

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