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Cell-Penetrating Peptides pp 417–429Cite as

Measuring the Action of CPP–siRNA Conjugates in the Lung

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 683))

Abstract

Two of the most promising and complex areas in biologics development, either as research tools or potential therapeutics, are cell-penetrating peptides (CPPs) and RNA interference (RNAi) modulators. Consequently, the combined application of these technologies in pursuit of improved delivery profiles for RNAi cargoes presents its own unique challenges. Direct access to the targeted tissue is luxury not always available to the researcher; however, the example of lung presents an excellent opportunity for presenting methodologies relevant to understanding the local impact of CPP-conjugated RNAi modulators. This chapter therefore expands upon updated protocols established on the study of the function of endogenous RNAi and the utility of CPPs in the delivery of short interfering RNA (siRNA) to therapeutically relevant cells in the lung. Methods for sample collection, preservation, and processing are provided with a view to facilitate qualitative and quantitative analysis of delivery. In addition, a protocol for mapping siRNA delivery by in situ hybridisation is provided.

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Author information

Authors and Affiliations

  1. Biotherapeutics, Pfizer Global Research and Development, Pfizer Inc, Sandwich, Kent, UK

    Sterghios A. Moschos

  2. Biotherapeutics, Pfizer Global Research and Development Pfizer Inc, Kent, UK

    Karen G. Spink

  3. NIHR Translational Research Facility in Respiratory Medicine, Wythenshawe Hospital, University of Manchester Education and Research Centre, Southmoor Road, Manchester, UK

    Mark A. Lindsay

Authors
  1. Sterghios A. Moschos
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  2. Karen G. Spink
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  3. Mark A. Lindsay
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Editor information

Editors and Affiliations

  1. , Department of Neurochemistry, Stockholm University, Svante Arrheniusv 21A21A, Stockholm, 106 91, Sweden

    Ülo Langel

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Moschos, S.A., Spink, K.G., Lindsay, M.A. (2011). Measuring the Action of CPP–siRNA Conjugates in the Lung. In: Langel, Ü. (eds) Cell-Penetrating Peptides. Methods in Molecular Biology, vol 683. Humana Press. https://doi.org/10.1007/978-1-60761-919-2_30

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  • DOI: https://doi.org/10.1007/978-1-60761-919-2_30

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-918-5

  • Online ISBN: 978-1-60761-919-2

  • eBook Packages: Springer Protocols

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