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Enhanced Cellular Delivery of Cell-Penetrating Peptide–Peptide Nucleic Acid Conjugates by Photochemical Internalization

  • Takehiko Shiraishi
  • Peter E. Nielsen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 683)

Abstract

Cell-penetrating peptides (CPPs) have been widely used for a cellular delivery of biologically relevant cargoes including antisense peptide nucleic acids (PNAs). Although chemical conjugation of PNA to a variety of CPPs significantly improves the cellular uptake of the PNAs, bioavailability (antisense activity) is still limited by endocytotic entrapment. We have shown that this low bioavailability can be greatly improved by combining CPP–PNA conjugate administration with a photochemical internalization technique using photosensitizers such as aluminum phthalocyanine (AlPcS2a) or tetraphenylporphyrin tetrasulfonic acid (TPPS). Cellular uptake of the PNA conjugates were evaluated by using a sensitive cellular method with HeLa pLuc705 cells based on the splicing correction of luciferase gene by targeting antisense oligonucleotides to a cryptic splice site of the mutated luciferase gene. The cellular efficacy of CPP conjugates were evaluated by measuring luciferase activity as a result of splicing correction and was also confirmed by RT-PCR analysis of luciferase pre-mRNA.

Key words

Antisense Cellular uptake Cell-penetrating peptide Peptide nucleic acid Photochemical internalization 

Notes

Acknowledgements

This work was supported by the Lundbeck Foundation, The Danish Council for Independent Research, and the NovoNordic Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Takehiko Shiraishi
    • 1
  • Peter E. Nielsen
    • 2
  1. 1.Department of Cellular and Molecular Medicine, Faculty of Health Sciences, The Panum InstituteUniversity of CopenhagenCopenhagenDenmark
  2. 2.Department of Cellular and Molecular Medicine, Faculty of Health, Sciences, The Panum InstituteUniversity of CopenhagenCopenhagen NDenmark

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