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Calcium and Membrane Repair

  • Caroline Palm-ApergiEmail author
  • Mattias Hällbrink
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 683)

Abstract

As more and more studies utilize cell-penetrating peptides to deliver pharmacologically interesting substances, there is a growing need to understand their effect on the plasma membrane. If a cell-penetrating peptide together with its cargo is to be used as a drug, it is necessary to understand how the conjugate interacts with the plasma membrane to enter the cell. A key regulator of the transportation network in the cell is calcium. This chapter describes five methods that can be employed for understanding how the plasma membrane reacts to the presence of cell-penetrating peptides and the involvement of calcium.

Key words

CPP PTD Membrane repair response FURA-2 Propidium iodide LAMP-2 

Notes

Acknowledgments

This work was supported by the Jeansson foundation.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of Cellular and Molecular MedicineUCSD School of Medicine, Howard Hughes Medical InstituteLa JollaUSA
  2. 2.Department NeurochemistryStockholm UniversityStockholmSweden

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