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Phenotypic and Functional Characterization of CD8+ T Regulatory Cells

  • Séverine Ménoret
  • Carole Guillonneau
  • Séverine Bezié
  • Lise Caron
  • Ignacio Anegon
  • Xian-Liang Li
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 677)

Abstract

Increasing evidence shows the presence and significance of CD8+ T regulatory cells (CD8+ Tregs) in both human and rodent transplant recipients, as well as in autoimmune disease models. We, hereafter, review all available data on the phenotypic and functional characterization of CD8+ Tregs, and we also provide detailed protocols to purify them and analyze their suppressive function. Different subsets of dendritic cells (DCs) and CD4+ effector T cells may modulate the suppression mediated by CD8+ Tregs. By analyzing the proliferation of CFSE-labeled naïve CD4+CD25− T cells in coculture MLR and transwell experiments, we explored the mutual modulation of CD8+ Tregs, DC subsets, and CD4+ T effector cells. The suppressive function of CD8+ Tregs was mediated by both cell-contact-dependent and -independent mechanisms.

Key words

CD8+ regulatory T cells CFSE Suppressive function Transwell analysis Dendritic cells CD4+ effector T cells 

Notes

Acknowledgments

This work was supported by a research fellowship from the Transplantation Society and the Senior Basic Science Grant from the European Society for Organ Transplantation (to X.L.L.) as well the grants from the Roche Organ Transplantation Research Foundation and the Fondation Transvie (to I.A.).

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Copyright information

© Humana Press 2010

Authors and Affiliations

  • Séverine Ménoret
    • 1
    • 2
    • 3
    • 4
  • Carole Guillonneau
    • 1
    • 2
    • 3
  • Séverine Bezié
    • 1
    • 2
    • 3
  • Lise Caron
    • 1
    • 2
    • 5
  • Ignacio Anegon
    • 1
    • 2
    • 3
  • Xian-Liang Li
    • 1
    • 2
    • 3
  1. 1.INSERM UMR 643NantesFrance
  2. 2.Institut de Transplantation et de Recherche en Transplantation (ITERT)CHU NantesNantesFrance
  3. 3.Faculté de MédecineUniversité de NantesNantesFrance
  4. 4.Platform Rat Transgenic Nantes IBiSA-CNRSNantesFrance
  5. 5.Université de NantesNantesFrance

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