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Methods for In Vitro Generation of Human Type 1 Regulatory T Cells

  • Silvia Gregori
  • Maria Grazia Roncarolo
  • Rosa BacchettaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 677)

Abstract

Type 1 regulatory T (Tr1) cells are adaptive regulatory T cells that are induced in the periphery upon chronic exposure to antigen (Ag) in a tolerogenic environment containing interleukin (IL)-10. Tr1 cells are Ag-specific; they produce high levels of IL-10 and TGF-β in the absence of IL-4 and suppress T-cell responses via a cytokine-dependent mechanism. During the last decade, several protocols have been developed to generate Tr1 cell lines in vitro. In this chapter, we outline protocols to generate non-Ag- and Ag-specific Tr1 cell lines and assays used to characterize Tr1 cell phenotype and functions.

Key words

Type 1 regulatory T cells Dendritic cells Interleukin-10 

Notes

Acknowledgments

The authors thank Claudia Sartirana and Chiara Francesca Magnani for technical help. This work was supported by a grant from the Italian Telethon Foundation, Cariplo Foundation, the Italian Association for Cancer Research (Associazione Italiana per la Ricerca sul Cancro, AIRC), and the EU graut to MGR, Riset Consortion (www.risetFp6.org)

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Copyright information

© Humana Press 2010

Authors and Affiliations

  • Silvia Gregori
    • 1
  • Maria Grazia Roncarolo
    • 1
    • 2
  • Rosa Bacchetta
    • 1
    Email author
  1. 1.Department of Regenerative Medicine, Stem Cells, and Gene TherapySan Raffaele Telethon Institute for Gene Therapy (HSR-TIGET)MilanItaly
  2. 2.Universita’ Vita-Salute San RaffaeleMilanItaly

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