In Vitro Analyses of the Immunosuppressive Properties of Neural Stem/Progenitor Cells Using Anti-CD3/CD28-Activated T Cells
Neural stem/progenitor cells (NSPCs) are multi-potent cells defined by their ability to self-renew and differentiate into cells of glial and neuronal lineage. Because of these properties, NSPCs have been proposed as therapeutic tools to replace lost neurons. Recent observations in animal models of immune-related diseases indicate that NSPCs display immunomodulatory properties that might be a great interest for cell therapy. In particular, transplantation of NSPCs might be very useful as local immunosuppressive agent to promote the long-term survival of neuronal xenotransplant in the brain. To study this possibility, we have analysed the impact of NSPCs on anti-CD3/CD28-activated T cells. In vitro analyses clearly show that porcine, rat, and mouse NSPCs inhibit the proliferation of activated T cells. This result raises new perspectives concerning the use of NSPCs in cell therapy.
Key wordsNeural progenitor Neural stem cell T cell Immunosuppression Culture Rat Pig Mouse
The authors are very grateful to Dr. I. Anegon and Dr. Vanhove for their helpful advices. We also gratefully acknowledge Dr. P. Brachet and Pr. J.-P. Soulillou for their support and encouragement. We also express special thanks to “Etablissement Français du Sang” (EFS, Nantes) that kindly irradiated the NSPCs. The Nestin monoclonal antibody was developed by Susan Hockfield and obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA 52242. The work was supported by the “Association Française contre les Myopathies” (AFM), the “Fédération des Groupements de Parkinsoniens”, and Progreffe. V.Bonnamain was supported by a fellowship from Ministère de l’Enseignement Supérieur et de la Recherche.
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