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Human Regulatory Macrophages

  • James A. Hutchinson
  • Paloma Riquelme
  • Edward K. Geissler
  • Fred Fändrich
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 677)

Abstract

Regulatory macrophages (M regs) are a novel type of suppressor macrophage which may be a particularly suitable cell for inducing tolerance of solid organ transplants. In this article, we provide a detailed description of the generation of human M regs from peripheral blood monocytes and methods for the assessment of their phenotype. The uniqueness of the human M reg is best appreciated when the M reg is compared to macrophages in other states of activation; therefore, protocols are provided for generating five comparator macrophage types which have been used as cell type-specificity controls in our work.

Key words

Suppressor macrophage Transplantation tolerance Cellular therapy 

References

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Copyright information

© Humana Press 2010

Authors and Affiliations

  • James A. Hutchinson
    • 1
  • Paloma Riquelme
    • 1
  • Edward K. Geissler
    • 1
  • Fred Fändrich
    • 2
  1. 1.Laboratory for Transplantation Research, Department of SurgeryUniversity Hospital RegensburgRegensburgGermany
  2. 2.Clinic for Applied Cell TherapyUniversity Hospital of Schleswig-HolsteinKielGermany

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