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Components and Mechanisms of Regulation of Gene Expression

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 674)

Abstract

The control of gene expression is a biological process essential to all organisms. This is accomplished through the interaction of regulatory proteins with specific DNA motifs in the control regions of the genes that they regulate. Upon binding to DNA, and through specific protein–protein interactions, these regulatory proteins convey signals to the basal transcriptional machinery, containing the respective RNA polymerases, resulting in particular rates of gene expression. In eukaryotes, in addition and complementary to the binding of regulatory proteins to DNA, chromatin structure plays a role in modulating gene expression. Small RNAs are emerging as key components in this process. This chapter provides an introduction to some of the basic players participating in these processes, the transcription factors and co-regulators, the cis-regulatory elements that often function as transcription factor docking sites, and the emerging role of small RNAs in the regulation of gene expression.

Key words

Promoter DNA-binding operon cis-regulatory element microRNA small interfering RNA 
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Notes

Acknowledgments

Support in the Grotewold lab for projects involving regulation of gene expression is provided by NRI Grant 2007-35318-17805 from the USDA CSREES, DOE Grant DE-FG02-07ER15881, and NSF grant DBI-0701405. A.Y. is supported by NIH Ruth L. Kirschstein National Research Service Award 5 T32 CA106196-05 from NCI.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Plant Biotechnology Center and Department of Plant Cellular and Molecular BiologyThe Ohio State UniversityColumbusUSA

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