Abstract
Circulating antibodies are highly selective binding reagents directed to a vast repertoire of antigens. Candidate antigens displayed as overlapping peptides on microarrays can be used to screen for recognition by serum antibodies from clinically well-defined patient populations. The methodology is robust and enables unbiased visualization of antigen-specific B-cell responses. Additionally, autoantibody signatures of diagnostic value could be detected using microarrays displaying thousands of human peptides.
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References
Nahtman, T., Jernberg, A., Mahdavifar, S., Zerweck, J., Schutkowski, M., Maeurer, M., and Reilly, M. (2007) Validation of peptide epitope microarray experiments and extraction of quality data, J Immunol Methods 328, 1–13.
Gaseitsiwe, S., Valentini, D., Mahdavifar, S., Magalhaes, I., Hoft, D. F., Zerweck, J., Schutkowski, M., Andersson, J., Reilly, M., and Maeurer, M. J. (2008) Pattern recognition in pulmonary tuberculosis defined by high content peptide microarray chip analysis representing 61 proteins from M. tuberculosis, PLoS One 3, e3840.
Ngo, Y., Advani, R., Valentini, D., Gaseitsiwe, S., Mahdavifar, S., Maeurer, M., and Reilly, M. (2009) Identification and testing of control peptides for antigen microarrays, J Immunol Methods 343, 68–78.
Gaseitsiwe, S., Valentini, D., Mahdavifar, S., Reilly, M., Ehrnst, A., and Maeurer, M. (2010) Peptide microarray – based identification of Mycobacterium tuberculosis epitope binding to HLA-DRB1*0101, DRB1*1501 and DRB1*0401, Clin Vaccine Immunol, 17(1):168–75.
Gaseitsiwe, S., Valentini, D., Ahmed, R., Mahdavifar, S., Magalhaes, I., Zerweck, J., Schutkowski, M., Gautherot, E., Montero, F., Ehrnst, A., Reilly, M., and Maeurer, M. (2009) Major histocompatibility complex class II molecule-human immunodeficiency virus peptide analysis using a microarray chip, Clin Vaccine Immunol 16, 567–573.
Melnyk, O., Duburcq, X., Olivier, C., Urbes, F., Auriault, C., and Gras-Masse, H. (2002) Peptide arrays for highly sensitive and specific antibody-binding fluorescence assays, Bioconjug Chem 13, 713–720.
Duburcq, X., Olivier, C., Malingue, F., Desmet, R., Bouzidi, A., Zhou, F., Auriault, C., Gras-Masse, H., and Melnyk, O. (2004) Peptide-protein microarrays for the simultaneous detection of pathogen infections, Bioconjug Chem 15, 307–316.
Bongartz, J., Bruni, N., and Or-Guil, M. (2009) Epitope mapping using randomly generated peptide libraries, Methods Mol Biol 524, 237–246.
Neuman de Vegvar, H. E., Amara, R. R., Steinman, L., Utz, P. J., Robinson, H. L., and Robinson, W. H. (2003) Microarray profiling of antibody responses against simian-human immunodeficiency virus: postchallenge convergence of reactivities independent of host histocompatibility type and vaccine regimen, J Virol 77, 11125–11138.
Neuman de Vegvar, H. E., and Robinson, W. H. (2004) Microarray profiling of antiviral antibodies for the development of diagnostics, vaccines, and therapeutics, Clin Immunol 111, 196–201.
Cerecedo, I., Zamora, J., Shreffler, W. G., Lin, J., Bardina, L., Dieguez, M. C., Wang, J., Muriel, A., de la Hoz, B., and Sampson, H. A. (2008) Mapping of the IgE and IgG4 sequential epitopes of milk allergens with a peptide microarray-based immunoassay, J Allergy Clin Immunol 122, 589–594.
Lin, J., Bardina, L., and Shreffler, W. G. (2009) Microarrayed allergen molecules for diagnostics of allergy, Methods Mol Biol 524, 259–272.
Lin, J., Bardina, L., Shreffler, W. G., Andreae, D. A., Ge, Y., Wang, J., Bruni, F. M., Fu, Z., Han, Y., and Sampson, H. A. (2009) Development of a novel peptide microarray for large-scale epitope mapping of food allergens, J Allergy Clin Immunol 124, 315–322, 322.e311–313.
Shreffler, W. G., Lencer, D. A., Bardina, L., and Sampson, H. A. (2005) IgE and IgG4 epitope mapping by microarray immunoassay reveals the diversity of immune response to the peanut allergen, Ara h 2, J Allergy Clin Immunol 116, 893–899.
Flinterman, A. E., Knol, E. F., Lencer, D., Bardina, L., den Hartog Jager, C. F., Lin, J., Pasmans, S. G., Bruijnzeel-Koomen, C. A., Sampson, H. A., van Hoffen, E., and Shreffler, W. G. (2008) Peanut epitopes for IgE and IgG4 in peanut-sensitized children in relation to severity of peanut allergy, J Allergy Clin Immunol. 121, 737–743.
Bar-Or, A., Vollmer, T., Antel, J., Arnold, D. L., Bodner, C. A., Campagnolo, D., Gianettoni, J., Jalili, F., Kachuck, N., Lapierre, Y., Niino, M., Oger, J., Price, M., Rhodes, S., Robinson, W. H., Shi, F. D., Utz, P. J., Valone, F., Weiner, L., Steinman, L., and Garren, H. (2007) Induction of antigen-specific tolerance in multiple sclerosis after immunization with DNA encoding myelin basic protein in a randomized, placebo-controlled phase 1/2 trial, Arch Neurol 64, 1407–1415.
Garren, H., Robinson, W. H., Krasulova, E., Havrdova, E., Nadj, C., Selmaj, K., Losy, J., Nadj, I., Radue, E. W., Kidd, B. A., Gianettoni, J., Tersini, K., Utz, P. J., Valone, F., and Steinman, L. (2008) Phase 2 trial of a DNA vaccine encoding myelin basic protein for multiple sclerosis, Ann Neurol 63, 611–620.
Lorenz, P., Kreutzer, M., Zerweck, J., Schutkowski, M., and Thiesen, H. J. (2009) Probing the epitope signatures of IgG antibodies in human serum from patients with autoimmune disease, Methods Mol Biol 524, 247–258.
Graham, K. L., Robinson, W. H., Steinman, L., and Utz, P. J. (2004) High-throughput methods for measuring autoantibodies in systemic lupus erythematosus and other autoimmune diseases, Autoimmunity 37, 269–272.
Robinson, W. H., Steinman, L., and Utz, P. J. (2002) Protein and peptide array analysis of autoimmune disease, Biotechniques Suppl, 66–69.
Robinson, W. H., Steinman, L., and Utz, P. J. (2003) Protein arrays for autoantibody profiling and fine-specificity mapping, Proteomics 3, 2077–2084.
Hueber, W., Zeng, D., Sharpe, O., Robinson, W. H., Strober, S., and Utz, P. J. (2004) Characterization of novel antigens recognized by serum autoantibodies from anti-CD1 TCR-transgenic lupus mice, Eur J Immunol 34, 1654–1662.
Quintana, F. J., Hagedorn, P. H., Elizur, G., Merbl, Y., Domany, E., and Cohen, I. R. (2004) Functional immunomics: microarray analysis of IgG autoantibody repertoires predicts the future response of mice to induced diabetes, Proc Natl Acad Sci U S A 101 Suppl 2, 14615–14621.
Hueber, W., Kidd, B. A., Tomooka, B. H., Lee, B. J., Bruce, B., Fries, J. F., Sonderstrup, G., Monach, P., Drijfhout, J. W., van Venrooij, W. J., Utz, P. J., Genovese, M. C., and Robinson, W. H. (2005) Antigen microarray profiling of autoantibodies in rheumatoid arthritis, Arthritis Rheum 52, 2645–2655.
Hueber, W., and Robinson, W. H. (2006) Proteomic biomarkers for autoimmune disease, Proteomics 6, 4100–4105.
Robinson, W. H., DiGennaro, C., Hueber, W., Haab, B. B., Kamachi, M., Dean, E. J., Fournel, S., Fong, D., Genovese, M. C., de Vegvar, H. E., Skriner, K., Hirschberg, D. L., Morris, R. I., Muller, S., Pruijn, G. J., van Venrooij, W. J., Smolen, J. S., Brown, P. O., Steinman, L., and Utz, P. J. (2002) Autoantigen microarrays for multiplex characterization of autoantibody responses, Nat Med 8, 295–301.
Kidd, B. A., Ho, P. P., Sharpe, O., Zhao, X., Tomooka, B. H., Kanter, J. L., Steinman, L., and Robinson, W. H. (2008) Epitope spreading to citrullinated antigens in mouse models of autoimmune arthritis and demyelination, Arthritis Res Ther 10, R119.
Britschgi, M., Olin, C. E., Johns, H. T., Takeda-Uchimura, Y., LeMieux, M. C., Rufibach, K., Rajadas, J., Zhang, H., Tomooka, B., Robinson, W. H., Clark, C. M., Fagan, A. M., Galasko, D. R., Holtzman, D. M., Jutel, M., Kaye, J. A., Lemere, C. A., Leszek, J., Li, G., Peskind, E. R., Quinn, J. F., Yesavage, J. A., Ghiso, J. A., and Wyss-Coray, T. (2009) Neuroprotective natural antibodies to assemblies of amyloidogenic peptides decrease with normal aging and advancing Alzheimer’s disease, Proc Natl Acad Sci U S A 106, 12145–12150.
Quintana, F. J., Farez, M. F., Viglietta, V., Iglesias, A. H., Merbl, Y., Izquierdo, G., Lucas, M., Basso, A. S., Khoury, S. J., Lucchinetti, C. F., Cohen, I. R., and Weiner, H. L. (2008) Antigen microarrays identify unique serum autoantibody signatures in clinical and pathologic subtypes of multiple sclerosis, Proc Natl Acad Sci U S A 105, 18889–18894.
Merbl, Y., Itzchak, R., Vider-Shalit, T., Louzoun, Y., Quintana, F. J., Vadai, E., Eisenbach, L., and Cohen, I. R. (2009) A systems immunology approach to the host-tumor interaction: large-scale patterns of natural autoantibodies distinguish healthy and tumor-bearing mice, PLoS One 4, e6053.
Falsey, J. R., Renil, M., Park, S., Li, S., and Lam, K. S. (2001) Peptide and small molecule microarray for high throughput cell adhesion and functional assays, Bioconjug Chem 12, 346–353.
Tapia, V., Bongartz, J., Schutkowski, M., Bruni, N., Weiser, A., Ay, B., Volkmer, R., and Or-Guil, M. (2007) Affinity profiling using the peptide microarray technology: a case study, Anal Biochem 363, 108–118.
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Masch, A., Zerweck, J., Reimer, U., Wenschuh, H., Schutkowski, M. (2010). Antibody Signatures Defined by High-Content Peptide Microarray Analysis. In: Uttamchandani, M., Yao, S. (eds) Small Molecule Microarrays. Methods in Molecular Biology, vol 669. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-845-4_13
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DOI: https://doi.org/10.1007/978-1-60761-845-4_13
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