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Construction and Screening of Marine Metagenomic Libraries

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 668)

Abstract

Marine microbial communities are highly diverse and have evolved during extended evolutionary processes of physiological adaptations under the influence of a variety of ecological conditions and selection pressures. They harbor an enormous diversity of microbes with still unknown and probably new physiological characteristics. Besides, the surfaces of marine multicellular organisms are typically covered by a consortium of epibiotic bacteria and act as barriers, where diverse interactions between microorganisms and hosts take place. Thus, microbial diversity in the water column of the oceans and the microbial consortia on marine tissues of multicellular organisms are rich sources for isolating novel bioactive compounds and genes. Here we describe the sampling, construction of large-insert metagenomic libraries from marine habitats and exemplarily one function based screen of metagenomic clones.

Key words

Isolation of metagenomic DNA 16S rDNA phylogenetic analysis Fosmid library Function-based screen 
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© Humana Press 2010

Authors and Affiliations

  1. 1.Institute for General MicrobiologyChristian Albrechts University KielKielGermany

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