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Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applications

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 666)

Abstract

Saliva is an ideal translational research tool and diagnostic medium and is being used in novel ways to provide molecular biomarkers for a variety of oral and systemic diseases and conditions. The ability to analyze saliva to monitor health and disease is a highly desirable goal for oral health promotion and research. Saliva has been used to detect caries risk, periodontitis, oral cancer, breast cancer, salivary gland diseases, and systemic disorders such as hepatitis, HIV and HCV. Technology advancement has allowed high-throughput studies to be performed at a scale unrealized previously and is serving to advance the discovery and validation of salivary disease biomarkers. Of course, successful measurement of salivary analytes requires optimal collection, processing, and storage procedures and conditions. This chapter describes protocols for saliva collection, processing, and storage for the molecular analysis of salivary diagnostic constituents.

Key words

Saliva saliva collection saliva processing diagnostics submandibular parotid sublingual 
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Notes

Acknowledgment

This research is supported by NIH grants UO1-DE016275, UO1-DE017790, and RO1-DE017170.

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© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.College of Dental Medicine, Western University of Health SciencesPomonaUSA
  2. 2.Division of Oral Biology and Oral MedicineUniversity of California Los AngelesLos AngelesUSA
  3. 3.Dental Research Institute, University of California Los AngelesLos AngelesUSA
  4. 4.School of Dentistry, University of California Los AngelesLos AngelesUSA
  5. 5.Department of Craniofacial BiologyUniversity of California Los AngelesLos AngelesUSA
  6. 6.Jonsson Comprehensive Cancer Center, University of California Los AngelesLos AngelesUSA
  7. 7.Molecular Biology InstituteUniversity of California Los AngelesLos AngelesUSA
  8. 8.Henry Samuel School of Engineering and Applied ScienceUniversity of California Los AngelesLos AngelesUSA

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