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Lyssaviruses: Special Emphasis on Rabies Virus and Other Members of the Lyssavirus Genus

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 665)

Abstract

Rabies is routinely diagnosed based on the clinical description and history of exposure in a rabies-endemic country. A negative diagnostic test for rabies virus or a related lyssavirus does not exclude the clinical diagnosis. Diagnostic tests are never optimal and are entirely dependent on the nature and quality of the sample supplied. Often, only a sample from a single time point is investigated reducing the overall sensitivity of any diagnosis. With the advent of molecular biology, tests have been developed that are rapid, robust, and sensitive in support of the rapid detection and strain identification of rabies virus from clinical specimens. These molecular tests complement conventional tests in rabies diagnosis, particularly for human cases, for which an early laboratory diagnosis is critical and may decrease the number of unnecessary contacts with the patient, reduce the requirement for invasive and costly interventions, and enable the appropriate medical treatment regimen to be administered for the patient. The barrier to success is in transferring the technology for the latest techniques in rabies diagnosis to rabies-endemic countries. These barriers are not insurmountable and in liaison with international organisations, especially OIE, FAO, and WHO, these diagnostic tests will be validated for rabies diagnosis and surveillance, and implemented in modern and well-equipped diagnostic laboratories throughout the world.

Key words

Rabies Lyssavirus RNA virus Zoonosis Diagnostic assay 
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Notes

Acknowledgements

GH and ARF are funded by the UK Department for Environment, Food, and Rural Affairs (Defra grant SEV3500).

References

  1. 1.
    Jackson, A.C. and Wunner, W.H. (2007) Rabies, 2nd Edn. Elsevier Inc, London, pp. 635–643.CrossRefGoogle Scholar
  2. 2.
    Johnson, N., Wade-Lipscomb, D., Stott, R., Rao, G.G., Mansfield, K., Smith, J., McElhinney, L.M., and Fooks, A.R. (2002) Investigation of a human case of rabies in the United Kingdom. J Clin Virol 25, 351–356.PubMedCrossRefGoogle Scholar
  3. 3.
    Noah, D.L., Drenzek, C.L., Smith, J.S., Krebs, J.W., Orciari, L., Shaddock, J., Sanderlin, D., Whitfield, S., Fekadu, M., Olson, J.G., Rupprecht, C.E., and Childs, J.E. (1998) Epidemiology of human rabies in the United States, 1980 to 1996. Ann Intern Med 128, 922–930.PubMedGoogle Scholar
  4. 4.
    Smith, J., McElhinney, L.M., Parsons, G., Brink, N., Doherty, T., Agranoff, D., Miranda, M.E., and Fooks, A.R. (2003) Case report: rapid antemortem diagnosis of a human case of rabies imported into the UK from the Philippines. J Med Virol 69, 150–155.PubMedCrossRefGoogle Scholar
  5. 5.
    Nathwani, D., McIntyre, P.G., White, K., Shearer, A.J., Reynolds, N., Walker, D., Orange, G.V., and Fooks, A.R. (2003) Fatal human rabies caused by European bat Lyssavirus type-2a infection in Scotland. Clin Infect Dis 37, 598–601.PubMedCrossRefGoogle Scholar
  6. 6.
    Bingham, J. and van der Merwe, M. (2002) Distribution of rabies antigen in infected brain material: determining the reliablility of different regions of the brain for the rabies fluorescent antibody test. J Virol Methods 101, 85–94.PubMedCrossRefGoogle Scholar
  7. 7.
    Meslin, F.-X., Kaplin, M.M., and Koprowski, H., (1996) Laboratory Techniques in Rabies, 4th Edn. World Health Organisation, Geneva, pp. 88–95.Google Scholar
  8. 8.
    Wright, E., Temperton, N.J., Marston, D., McElhinney, L.M., Fooks, A.R., and Weiss, R.A. (2008) Investigating antibody neutralization of lyssaviruses using lentiviral pseudotypes: a cross-species comparison. J Gen Virol 89, 1–10.CrossRefGoogle Scholar
  9. 9.
    Brookes, S.M., Healy, D.M., and Fooks, A.R. (2006) Ability of rabies vaccine strains to elicit cross-neutralising antibodies. Dev Biol (Basel) 125, 185–193.Google Scholar
  10. 10.
    Dacheux, L., Reynes, J.-M., Buchy, P., Sivuth, O., Diop, B.M., Rousset, D., Rathat, C., Jolly, N., Dufourcq, J.-B., Nareth, C., Diop, C., Lehle, C., Rajerison, R., Sadorge, C., and Bourhy, H. (2008) A reliable diagnosis of human rabies based on analysis of skin biopsy specimens. Clin Infect Dis 47, 1410–1417.PubMedCrossRefGoogle Scholar
  11. 11.
    Cliquet, F., Aubert, M., and Sagne L. (1998) Development of a fluorescent antibody virus neutralisation test (FAVN test) for the quantitation of rabies-neutralising antibody. J Immunol Methods 212, 79–87.PubMedCrossRefGoogle Scholar
  12. 12.
    Picard-Meyer, E. and Cliquet, F. (2007) Use of filter paper (FTA®) technology for sampling, recovery and molecular characterisation of rabies viruses. J Virol Methods 140, 174–182.PubMedCrossRefGoogle Scholar
  13. 13.
    Black, E.M., Lowings, J.P., Smith, J., Heaton, P.R., and McElhinney, L.M. (2003) Corrigendum: a rapid RT-PCR method to differentiate six established genotypes of rabies and rabiesrelated viruses using TaqMan (TM) technology. J Virol Methods 111, 163.CrossRefGoogle Scholar
  14. 14.
    Heaton, P.R., Johnstone, P., McElhinney, L.M., Cowley, R., O’Sullivan, E., and Whitby, J. (1997) Hemi-nested PCR assay for detection of six genotypes of rabies and rabies-related viruses. J Clin Microbiol 35(11), 2762–2766.PubMedGoogle Scholar
  15. 15.
    Wakeley, P.R., Johnson, N., McElhinney, L.M., Marston, D., Sawyer, J., and Fooks, A.R. (2005) Development of a real-time, TaqMan reverse transcription-PCR assay for detection and differentiation of lyssavirus genotypes 1, 5 and 6. J Clin Microbiol 43, 2786–2792.PubMedCrossRefGoogle Scholar
  16. 16.
    King, A.A. (1993) Monoclonal antibody studies on rabies-related viruses. Onderstepoort J Vet Res 60, 283–287.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Rabies and Wildlife Zoonoses GroupVeterinary Laboratories AgencySurreyUK

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