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Structural Studies of MAP Kinase Cascade Components

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Book cover MAP Kinase Signaling Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 661))

Abstract

MAPK cascade components have been the subject of structural analysis, advancing our understanding of how these enzymes are activated and how they interact. A surprising finding has been that unique inactive conformers are adopted by many of these kinases. These inactive conformers are interesting and often require experimental phases to determine their crystal structures because molecular replacement techniques are not successful. Here, we describe the preparation of MAP2K MEK6 and MAP3K TAO2 substituted with selenomethionine (SeMet) for de novo phasing. TAO2 and SeMet TAO2 were expressed in insect cells.

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Acknowledgments

Our thanks go to Thomas Moon and Saurabh Mendiratta for assistance in preparing Table 1. Our work in this area has been supported by an NIH grant DK46003 and a grant from the Welch Foundation, I1128.

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  1. Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA

    Elizabeth J. Goldsmith, Xiaoshan Min, Haixia He & Tianjun Zhou

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  1. Elizabeth J. Goldsmith
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  2. Xiaoshan Min
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Correspondence to Elizabeth J. Goldsmith .

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  1. Dept. Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

    Rony Seger

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Goldsmith, E.J., Min, X., He, H., Zhou, T. (2010). Structural Studies of MAP Kinase Cascade Components. In: Seger, R. (eds) MAP Kinase Signaling Protocols. Methods in Molecular Biology, vol 661. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-795-2_13

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  • DOI: https://doi.org/10.1007/978-1-60761-795-2_13

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