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FISH on Chromosomes Derived from the Snail Model Organism Biomphalaria glabrata

  • Edwin C. Odoemelam
  • Nithya Raghavan
  • Wannaporn Ittiprasert
  • Andre Miller
  • Joanna M. Bridger
  • Matty KnightEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 659)

Abstract

The application of fluorescence in situ hybridization (FISH) for the mapping of single copy genes onto homologous chromosome has been integral to vast number genome sequencing projects, such as that of mouse and human. The chromosomes of these organisms are well-studied and are the staple resource of most of the early studies conducted in cytogenetics. However, there are now protocols for analyzing FISH probes in a number of different organisms on both metaphase and interphase chromosomes.

Here, we describe the methodologies for the chromosomal mapping of nonrepetitive (single-copy) genes of the snail Biomphalaria glabrata onto metaphase chromosomes derived from the only molluscan cell-line in existence. The technique described in this chapter was developed for the B. glabrata genome sequencing project through troubleshooting experimental procedures established for other organisms so that both the optimum resolution of metaphase chromosome and the effective hybridization of genes were achieved.

Key words

Fluorescence in situ hybridization Snail chromosomes Biomphalaria glabrata Bge embryonic cell line Molluscan Planorbid gastropod 

Notes

Acknowledgement

This study was funded by a grant from NIH-NIAID, RO1-AI63480.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Edwin C. Odoemelam
    • 1
  • Nithya Raghavan
    • 2
  • Wannaporn Ittiprasert
    • 2
  • Andre Miller
    • 2
  • Joanna M. Bridger
    • 3
  • Matty Knight
    • 2
    Email author
  1. 1.Division of BiosciencesBrunel UniversityMiddlesexUK
  2. 2.Biomedical Research InstituteRockvilleUSA
  3. 3.Laboratory of Nuclear and Genomic Health, Centre for Cell and Chromosome Biology, Division of Biosciences, School of Health Sciences and Social CareBrunel UniversityMiddlesexUK

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