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FISH as a Tool to Investigate Chromosome Behavior in Budding Yeast

  • Harry ScherthanEmail author
  • Josef Loidl
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 659)

Abstract

Fluorescence in situ hybridization (FISH) provides an effective means to delineate chromosomes and their subregions during all stages of the cell cycle. This makes FISH particularly useful for studying chromosome behavior in species with minute genomes and/or poor chromosome condensation at metaphase, which is the case in model organisms such as the budding yeast Saccharomyces cerevisiae. Since its introduction in 1992, FISH with composite whole chromosome or locus specific probes has become an indispensable tool in the analysis of chromosome behavior in metaphase and interphase cells, and especially of meiotic chromosome pairing of wild-type and mutant yeast strains.

Key words

DNA labeling FISH GISH Immunofluorescence Saccharomyces cerevisiae Chromosome painting Chromosome dynamics 

Notes

Acknowledgments

We thank A. Lorenz, J. Fuchs, and E. Trelles-Sticken for insightful comments and stimulating discussions. The work in the lab of HS was partly supported by the DFG (SCHE 350/10-1, SPP 1384) and H.-H. Ropers, Max-Planck-Inst. for Molecular Genetics, Berlin, FRG.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Institut für Radiobiologie der BundeswehrMunichGermany
  2. 2.Max Planck Institute for Molecular GeneticsBerlinGermany
  3. 3.Department of Chromosome BiologyUniversity of ViennaViennaAustria

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