FISH as a Tool to Investigate Chromosome Behavior in Budding Yeast
Fluorescence in situ hybridization (FISH) provides an effective means to delineate chromosomes and their subregions during all stages of the cell cycle. This makes FISH particularly useful for studying chromosome behavior in species with minute genomes and/or poor chromosome condensation at metaphase, which is the case in model organisms such as the budding yeast Saccharomyces cerevisiae. Since its introduction in 1992, FISH with composite whole chromosome or locus specific probes has become an indispensable tool in the analysis of chromosome behavior in metaphase and interphase cells, and especially of meiotic chromosome pairing of wild-type and mutant yeast strains.
Key wordsDNA labeling FISH GISH Immunofluorescence Saccharomyces cerevisiae Chromosome painting Chromosome dynamics
We thank A. Lorenz, J. Fuchs, and E. Trelles-Sticken for insightful comments and stimulating discussions. The work in the lab of HS was partly supported by the DFG (SCHE 350/10-1, SPP 1384) and H.-H. Ropers, Max-Planck-Inst. for Molecular Genetics, Berlin, FRG.
- 2.The Yeast Genome Directory. (1997). Nature 387, 1–105.Google Scholar
- 16.Admire, A., Shanks, L., Danzl, N., Wang, M., Weier, U., Stevens, W., Hunt, E., and Weinert, T. (2006) Cycles of chromosome instability are associated with a fragile site and are increased by defects in DNA replication and checkpoint controls in yeast. Genes Dev 20, 159–173.PubMedCrossRefGoogle Scholar
- 27.Scherthan, H. and Trelles-Sticken, E. (2002) Yeast FISH: delineation of chromosomal targets in vegetative and meiotic yeast cells, in FISH Technology, Springer Lab Manual (Rautenstrauss, B., and Liehr, T., eds.), Springer, Heidelberg, New York, pp. 329–346.Google Scholar