Abstract
Fluorescence in situ hybridization (FISH) is widely used for the localization of genes and specific genomic regions on target chromosomes, both in metaphase and interphase cells. The applications of FISH are not limited to gene mapping or the study of genetic rearrangements in human diseases. Indeed, FISH is increasingly used to explore the genome organization in various organisms and extends to the study of animal and plant biology. We have described the principles and basic methodology of FISH to be applied to the study of metaphase and interphase chromosomes.
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References
Pinkel, D., Straume, T., and Gray, J.W. (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83, 2834–2938.
Saunders, K., Czepulkowski, B. (2001) Culture of human cells for chromosomal analysis, in Analyzing Chromosomes (Czepulkowski, B., ed.), BIOS Scientific Publishers, Oxford, UK, pp 19–68.
Kearney, L., Tosi, S., and Jafu, R.J. (2002) Detection of chromosome abnormalities in leukemia using fluorescence in situ hybridization, in Methods in molecular medicine, vol. 68: molecular analysis of cancer (Boultwood, J., and Fidler, C., eds.), Humana, Totowa, NJ, pp 14–15.
Spurbeck, J.L., Zinsmeister, A.R., Meyer, K.J., and Jalal, S.M. (1996) Dynamics of chromosomes spreading. Am J Med Genet 61, 387–393.
Jauch, A., Daumer, C., Lichter, P., Murken, J., Schroeder-Kurt, T., and Cremer, T. (1990) Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics. Hum Genet 85, 145–150.
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Garimberti, E., Tosi, S. (2010). Fluorescence in situ Hybridization (FISH), Basic Principles and Methodology. In: Bridger, J., Volpi, E. (eds) Fluorescence in situ Hybridization (FISH). Methods in Molecular Biology, vol 659. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-789-1_1
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DOI: https://doi.org/10.1007/978-1-60761-789-1_1
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-60761-789-1
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