Fluorescence in situ Hybridization (FISH), Basic Principles and Methodology

  • Elisa Garimberti
  • Sabrina TosiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 659)


Fluorescence in situ hybridization (FISH) is widely used for the localization of genes and specific genomic regions on target chromosomes, both in metaphase and interphase cells. The applications of FISH are not limited to gene mapping or the study of genetic rearrangements in human diseases. Indeed, FISH is increasingly used to explore the genome organization in various organisms and extends to the study of animal and plant biology. We have described the principles and basic methodology of FISH to be applied to the study of metaphase and interphase chromosomes.

Key words

Chromosomes DNA probes Epifluorescence microscopy Fluorescence in situ Hybridization Interphase nuclei 


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Laboratory of Leukaemia and Chromosome Research, Division of Biosciences, Centre for Cell & Chromosome Biology, Brunel Institute for Cancer Genetics and PharmacogenomicsBrunel UniversityMiddlesexUK

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